منابع مشابه
Advantage of combining magnetic cell separation with sperm preparation techniques.
The selection of vital, non-apoptotic spermatozoa is a prerequisite for achieving optimal conception rates in assisted reproductive techniques. Magnetic cell sorting using annexin-V microbeads can effectively separate apoptotic and non-apoptotic spermatozoa. The objective of the present study was to optimize the integration of magnetic cell sorting in standard sperm preparations and to correlat...
متن کاملEffects of semen storage and separation techniques on sperm DNA fragmentation.
OBJECTIVE To determine the effect of semen storage and separation techniques on sperm DNA fragmentation. DESIGN Controlled clinical study. SETTING An assisted reproductive technology laboratory. PATIENT(S) Thirty normoozospermic semen samples obtained from patients undergoing infertility evaluation. INTERVENTION(S) One aliquot from each sample was immediately prepared (control) for the ...
متن کاملSperm Cell Separation Using a Spiral Channel
This work presents a specifically designed spiral type inertial micro-fluidic channel for separating non-motile sperm cell from cellular debris. Demonstration with a mixture of non-motile sperm and red blood cells showed that 82% of sperm could be focused into a separate outlet from the red blood cells.
متن کاملSeparation techniques: Chromatography
Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. Proteins can be purified based on characteristics such as size and shape, total charge, hydrophobic groups present on the surface, and binding capacity with the stationary phase. Four separation techniques ba...
متن کاملSeparation Using Foaming Techniques
Separation of various chemical components from each otheJO is often the most difficult step in analytical procedures. The problems attached to separation become further magnified when the species concentations are extremely low. A group of techniques that has proven useful especially in dilute solutions for separating and concentrating metallic as well as nonmetallic ions and complexes, protein...
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ژورنال
عنوان ژورنال: International Journal of Andrology
سال: 1987
ISSN: 0105-6263,1365-2605
DOI: 10.1111/j.1365-2605.1987.tb00364.x